Journal: Cell host & microbe

Article Title: IFI16 targets the transcription factor Sp1 to suppress HIV-1 transcription and latency reactivation

doi: 10.1016/j.chom.2019.05.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 2.1 TaKaRa Cat# 6022 GalScreen Applied Bioscience Cat# T1027 RNeasy Plus Mini Kit QIAGEN Cat# 74136 DNA-freeTM DNA Removal Kit ThermoFisher Cat# AM1906 PrimeScript RT Reagent Kit TAKARA Cat# RR037A TaqManTM Fast Universal PCR Master Mix ThermoFisher Cat# 4352042 GAPDH Endogenous Control (VIC/TAMRA) ThermoFisher Cat# 4310884E Luciferase Cell Culture Lysis 5X Reagent Promega Cat# E1531 Luciferase Assay System 10-pack Promega Cat# E1501 Coelenterazine (native-CTZ) p.j.k.

Techniques: Virus, Recombinant, Purification, Transfection, Enzyme-linked Immunosorbent Assay, Cell Isolation, Mutagenesis, DNA Ligation, Control, Luciferase, Cell Culture, Lysis, Magnetic Beads, Bicinchoninic Acid Protein Assay, Transcription Factor Assay, Activity Assay, Expressing, Construct, Plasmid Preparation, Binding Assay, Generated, Software

Journal: Cell host & microbe

Article Title: IFI16 targets the transcription factor Sp1 to suppress HIV-1 transcription and latency reactivation

doi: 10.1016/j.chom.2019.05.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 2.1 TaKaRa Cat# 6022 GalScreen Applied Bioscience Cat# T1027 RNeasy Plus Mini Kit QIAGEN Cat# 74136 DNA-freeTM DNA Removal Kit ThermoFisher Cat# AM1906 PrimeScript RT Reagent Kit TAKARA Cat# RR037A TaqManTM Fast Universal PCR Master Mix ThermoFisher Cat# 4352042 GAPDH Endogenous Control (VIC/TAMRA) ThermoFisher Cat# 4310884E Luciferase Cell Culture Lysis 5X Reagent Promega Cat# E1531 Luciferase Assay System 10-pack Promega Cat# E1501 Coelenterazine (native-CTZ) p.j.k.

Techniques: Virus, Recombinant, Purification, Transfection, Enzyme-linked Immunosorbent Assay, Cell Isolation, Mutagenesis, DNA Ligation, Control, Luciferase, Cell Culture, Lysis, Magnetic Beads, Bicinchoninic Acid Protein Assay, Transcription Factor Assay, Activity Assay, Expressing, Construct, Plasmid Preparation, Binding Assay, Generated, Software

Journal: Molecular medicine reports

Article Title: Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway.

doi: 10.3892/mmr.2013.1668

Figure Lengend Snippet: Figure 4. Differentiation of mesenchymal stem cells by FGF2 was suppressed through the mitogen‑activated protein kinase pathway. (A) Cell lysates were collected and efficacy of overexpression was determined by sodium dodecyl sulfate‑polyacrylamide gel electrophoresis and immunoblot analysis with antibodies to Spry1 and 4. (B) Expression of FGF2, p-ERK, ERK, Coll I, Coll III and scleraxis were analyzed by western blot analysis. GAPDH served as the loading control. FGF2, fibroblast growth factor 2; p-ERK, phos phorylated extracellular signal‑regulated kinase; Coll, collagen; GAPDH, glyceraldehyde 3‑phosphate dehydrogenase.

Article Snippet: RNA quality was assessed by the Blue Pippin automated electrophoresis system (Sage Science, Inc., Beverly, MA, USA) using the Experion RNA StdSens Analysis kit (Bio-Rad, Hercules, CA, USA). cDNA was prepared using the PrimeScript II First Strand cDNA Synthesis kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer's instructions, and 1 μl 5X diluted cDNA was used for further gene amplification. qPCR was performed by FastStart Universal SYBR-Green Master (Rox; Roche Dianostics, Mannheim, Germany).

Techniques: Over Expression, Nucleic Acid Electrophoresis, Western Blot, Expressing, Control